Coding

Part:BBa_K4611010:Design

Designed by: Saiyu Luo   Group: iGEM23_CJUH-JLU-China   (2023-10-03)


LwaCas13a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2830
    Illegal BglII site found at 1286
    Illegal BglII site found at 1496
    Illegal BglII site found at 1763
    Illegal BglII site found at 2528
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Leptotrichia wadei genome, but is human codon optimized. Provided by pC013-Twinstrep-SUMO-huLwCas13a(addgene).

References

[1] Yang J, Song Y, Deng X, Vanegas JA, You Z, Zhang Y, Weng Z, Avery L, Dieckhaus KD, Peddi A, Gao Y, Zhang Y, Gao X. Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection. Nat Chem Biol. 2023 Jan;19(1):45-54. doi: 10.1038/s41589-022-01135-yIF: 14.8 Q1 . Epub 2022 Sep 22. PMID: 36138140.